An EH Shepard illustration showing Winnie the Pooh and Cristopher Robin playing Pooh Sticks has sold for £314,500 ($492,727) at Sotheby's.
The lot, titled For a Long Time They Looked at the River Beneath Them, headlined the English Literature sale in London on December 9 - achieving a 109.6% increase on a £150,000 ($235,005) estimate.
It set a new record for an illustration from a book at auction.
The iconic drawing first appears in AA Milne's first collection of stories starring the titular bear, The House at Pooh Corner (1928).
Text accompanying the image reads: "For a long time they looked at the river beneath them, saying nothing, and the river said nothing too, for it felt very quiet and peaceful on this summer afternoon."
The much-loved series of books have an international following, with the result that manuscripts and illustrations sell very well at auction.
A further three Shepard illustrations in the sale sold for sums considerably above their estimates.
An autograph manuscript of poems by Dylan Thomas also sold well, realising £104,500 ($163,720)
The lot contains early drafts of some of his most famous works.
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